The BioInsights Podcast
The BioInsights Podcast: discussing the challenges of translating novel biologics from bench to clinic to market.
The BioInsights Podcast
Enhancing mRNA results through strategic RNA design and quality control
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BioInsights
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Season 4
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Episode 3
In the last few years, mRNA has moved to the fore in the vaccine and cell and gene therapy fields, and as a result, its successful production for these downstream applications has never been more crucial. In this podcast episode, Róisin McGuigan, Editor, Nucleic Acid Insights, speaks to Ryan Lahr, Senior R&D Scientist, CELLSCRIPT, about design, production, and quality control of synthesized mRNA for translation in cells.
Can you frame for us the key challenges and considerations when optimizing mRNA design for different research applications in the vaccine and cell and gene therapy fields?
Can you expand on the impact design decisions around nucleoside composition, cap structure and length of poly(A) tail have, on both the quality and functionality of mRNA?
What advice would you share with developers looking to optimize mRNA design and synthesis for their own specific application?
What are the key considerations and common pitfalls when developing a quality control strategy for synthesized transcripts? What analytical tools and techniques are available to assess the quality of mRNA transcripts?
And finally, what advice would you give to new researchers interested in using mRNA for translation in cells?